A Paradigm Shift: AUC As A Multi-Attribute Platform Method In Targeted Protein Degradation

Targeted protein degradation (TPD) is reshaping the future of drug discovery. Unlike traditional occupancy-driven therapies that inhibit protein function, TPD modalities use small molecules to hijack the cell’s own degradation machinery, achieving event-driven clearance of target proteins. This elegant mechanism has captured the attention of drug developers worldwide, but it also presents unique analytical challenges. Understanding ternary complex formation between the target protein, degrader molecule, and E3 ubiquitin ligase is critical to advancing these therapeutics from bench to clinic.

Watch Alexander E. Yarawsky, PhD, Principal Scientist at BioAnalysis, demonstrate how analytical ultracentrifugation (AUC) is uniquely positioned to characterize these complex interactions. Dr. Yarawsky introduces the Velocity Assay for Ternary Complex (VATC), a powerful application of AUC that enables compound screening while simultaneously revealing mechanistic insights. Having pioneered innovative AUC methodologies for gene therapy and protein characterization, including the development of BASIS™, BioAnalysis’s proprietary cGMP software platform, Dr. Yarawsky shows how rigorous global fitting of AUC data extracts thermodynamic and kinetic parameters that drive rational compound optimization. When combined with hydrodynamic modeling, AUC provides structural understanding of protein-degrader interactions that complement traditional structural biology approaches. This presentation exemplifies BioAnalysis’s commitment to scientific excellence, bringing cutting-edge biophysical expertise to one of drug discovery’s most promising frontiers.

Whether you’re in early discovery screening TPD compounds or advancing lead candidates toward regulatory submission, this session explores how AUC serves as a multi-attribute platform method for targeted protein degradation programs.

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Presentation Details: September 23, 2025
Originally presented 10:00 AM ET / 4:00 PM CET